pMCSG7-D16A-NmeCas9
(Plasmid
#71476)
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Purposebacterial pMCSG7 expression vector expressing Nme cas9 nickase with a D16A mutation (RuvC domain) with T7 promoter, N-terminal His tag and TEV site
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 71476 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMCSG7
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Backbone manufacturerMCSG
- Backbone size w/o insert (bp) 5286
- Total vector size (bp) 8541
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namepMCSG7-D16A-NmeCas9
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SpeciesNeisseria meningitidis
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Insert Size (bp)3321
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MutationD16A
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Tag
/ Fusion Protein
- 6His (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMCSG7-D16A-NmeCas9 was a gift from Erik Sontheimer (Addgene plasmid # 71476 ; http://n2t.net/addgene:71476 ; RRID:Addgene_71476) -
For your References section:
DNase H Activity of Neisseria meningitidis Cas9. Zhang Y, Rajan R, Seifert HS, Mondragon A, Sontheimer EJ. Mol Cell. 2015 Oct 15;60(2):242-55. doi: 10.1016/j.molcel.2015.09.020. 10.1016/j.molcel.2015.09.020 PubMed 26474066
Map uploaded by the depositor.
