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Addgene

pET28a-HsSENP3
(Plasmid #71467)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71467 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5329
  • Total vector size (bp) 7054
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SUMO1/sentrin specific peptidase 3
  • Alt name
    SENP3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1725
  • Mutation
    Gene optimised for E. coli expression (Genscript)
  • GenBank ID
    NM_015670.5
  • Entrez Gene
    SENP3 (a.k.a. SMT3IP1, SSP3, Ulp1)
  • Promoter T7
  • Tag / Fusion Protein
    • Hexahistidine (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-HsSENP3 was a gift from Jorge Eduardo Azevedo (Addgene plasmid # 71467 ; http://n2t.net/addgene:71467 ; RRID:Addgene_71467)

  • For your References section:

    Evaluation of the activity and substrate specificity of the human SENP family of SUMO proteases. Mendes AV, Grou CP, Azevedo JE, Pinto MP. Biochim Biophys Acta. 2015 Oct 29. pii: S0167-4889(15)00373-0. doi: 10.1016/j.bbamcr.2015.10.020. 10.1016/j.bbamcr.2015.10.020 PubMed 26522917
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