pET28a-HsSENP1
(Plasmid #71465)

• Purpose: Encodes human full length SENP1 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).
• Depositing Lab: Jorge Eduardo Azevedo
• Publication: Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii: S0167-4889(15)00373-0. doi: 10.1016/j.bbamcr.2015.10.020.

Backbone

• Vector backbone: pET-28a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5289
• Total vector size (bp): 7221
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: SUMO1/sentrin specific peptidase 1
• Alt name: SENP1
• Species: H. sapiens (human)
• Insert Size (bp): 1932
• Mutation: Gene optimised for E. coli expression (Genscript)
• GenBank ID: NM_001267594.1
• Entrez Gene: SENP1 (a.k.a. SuPr-2)
• Promoter: T7
• Tag / Fusion Protein:
  • Hexahistidine (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7term

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insert gene optimisation performed by Genscript