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Addgene

pcDNA-HA-TRAF3IP3 (mouse)
(Plasmid #71459)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71459 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA-HA
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TRAF3IP3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1545
  • Entrez Gene
    Traf3ip3 (a.k.a. 6030423D04Rik, AI115021, T3jam)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (destroyed during cloning)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-HA-TRAF3IP3 (mouse) was a gift from Shao-Cong Sun (Addgene plasmid # 71459 ; http://n2t.net/addgene:71459 ; RRID:Addgene_71459)

  • For your References section:

    T cell development involves TRAF3IP3-mediated ERK signaling in the Golgi. Zou Q, Jin J, Xiao Y, Hu H, Zhou X, Jie Z, Xie X, Li JY, Cheng X, Sun SC. J Exp Med. 2015 Jul 27;212(8):1323-36. doi: 10.1084/jem.20150110. Epub 2015 Jul 20. 10.1084/jem.20150110 PubMed 26195727
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