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Addgene

pCLXSN(GFP)-HA-Slc1a5 (mouse)
(Plasmid #71458)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71458 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCLXSN-GFP
  • Backbone manufacturer
    Obtained from Dr. Inder Verma and modified in Dr. Shao-Cong's lab. Addgene Plasmid #27557
  • Backbone size w/o insert (bp) 8000
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Slc1a5
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1650
  • Mutation
    A85P, L125F and V384A mutations compared to GenBank reference sequence NM_009201.2
  • Entrez Gene
    Slc1a5 (a.k.a. AAAT, ASCT2, ATBO, M7V1, M7VS1, R16, RDRC, Slc1a7)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CCT CAC TCC TTC TCT AGG CG (or pLXSN 5')
  • 3′ sequencing primer CAC CCT AAC TGA CAC ACA TTC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

We transferred the Slc1a5 insert from the pCLXSN vector. The differences in Slc1a5 compared to GenBank reference sequence NM_009201.2 do not affect the function of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCLXSN(GFP)-HA-Slc1a5 (mouse) was a gift from Shao-Cong Sun (Addgene plasmid # 71458 ; http://n2t.net/addgene:71458 ; RRID:Addgene_71458)

  • For your References section:

    Inflammatory T cell responses rely on amino acid transporter ASCT2 facilitation of glutamine uptake and mTORC1 kinase activation. Nakaya M, Xiao Y, Zhou X, Chang JH, Chang M, Cheng X, Blonska M, Lin X, Sun SC. Immunity. 2014 May 15;40(5):692-705. doi: 10.1016/j.immuni.2014.04.007. Epub 2014 May 1. 10.1016/j.immuni.2014.04.007 PubMed 24792914
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