pLEGFP-K685R-STAT3
(Plasmid #71449)

• Purpose: Retroviral expression of K685R-STAT3. Please note that this plasmid contains K685R-STAT3 tagged with FLAG, not GFP.
• Depositing Lab: George Stark
• Publication: Dasgupta et al Proc Natl Acad Sci U S A. 2015 Mar 31;112(13):3985-90. doi: 10.1073/pnas.1503152112. Epub 2015 Mar 12.

Backbone

• Vector backbone: pLEGFP-N1-neo
• Backbone manufacturer: Clontech
• Vector type: Retroviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: STAT3
• Species: H. sapiens (human)
• Mutation: K685R
• Entrez Gene: STAT3 (a.k.a. ADMIO, ADMIO1, APRF, HIES)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EGFP-N

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry