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Addgene

pLEGFP-S727A-STAT3
(Plasmid #71446)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71446 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLEGFP-N1-neo
  • Backbone manufacturer
    Clontech
  • Vector type
    Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    STAT3
  • Species
    H. sapiens (human)
  • Mutation
    S727A
  • Entrez Gene
    STAT3 (a.k.a. ADMIO, ADMIO1, APRF, HIES)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EGFP-N
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLEGFP-S727A-STAT3 was a gift from George Stark (Addgene plasmid # 71446 ; http://n2t.net/addgene:71446 ; RRID:Addgene_71446)

  • For your References section:

    STAT3-driven transcription depends upon the dimethylation of K49 by EZH2. Dasgupta M, Dermawan JK, Willard B, Stark GR. Proc Natl Acad Sci U S A. 2015 Mar 31;112(13):3985-90. doi: 10.1073/pnas.1503152112. Epub 2015 Mar 12. 10.1073/pnas.1503152112 PubMed 25767098
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