pcDNA3-­RAGEdeltaCyto
(Plasmid #71436)

• Purpose: mammalian expression of human RAGE cytoplasmic domain mutant
• Depositing Lab: Henri Huttunen
• Publication: Huttunen et al J Biol Chem. 1999 Jul 9;274(28):19919-24.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Total vector size (bp): 6640
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RAGE
• Alt name: Receptor for Advanced Glycation End Products
• Alt name: advanced glycosylation end product-specific receptor
• Species: H. sapiens (human)
• Mutation: delta 367-404
• Entrez Gene: AGER (a.k.a. RAGE, SCARJ1, sRAGE)
• Promoter: CMV

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry