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Addgene

pcDNA3-­RAGE
(Plasmid #71435)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71435 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Total vector size (bp) 6640
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RAGE
  • Alt name
    Receptor for Advanced Glycation End Products
  • Alt name
    advanced glycosylation end product-specific receptor
  • Species
    H. sapiens (human)
  • Entrez Gene
    AGER (a.k.a. RAGE, SCARJ1, sRAGE)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI/XhoI (destroyed during cloning)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGHrev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-­RAGE was a gift from Henri Huttunen (Addgene plasmid # 71435 ; http://n2t.net/addgene:71435 ; RRID:Addgene_71435)

  • For your References section:

    Receptor for advanced glycation end products (RAGE)-mediated neurite outgrowth and activation of NF-kappaB require the cytoplasmic domain of the receptor but different downstream signaling pathways. Huttunen HJ, Fages C, Rauvala H. J Biol Chem. 1999 Jul 9;274(28):19919-24. PubMed 10391939
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