pmGFP10C-­Tau
(Plasmid #71433)

• Purpose: Split GFP plasmid for visualization of Tau dimerization. Used together with plasmid 71434
• Depositing Lab: Henri Huttunen
• Publication: Brunello et al Sci Rep. 2016 Jul 27;6:30498. doi: 10.1038/srep30498.

Backbone

• Vector backbone: pmGFP10C
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Tau (0N4R)
• Species: H. sapiens (human)
• Entrez Gene: MAPT (a.k.a. DDPAC, FTD1, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, Tau-PHF6, tau-40)
• Tag / Fusion Protein:
  • GFP (1-212) (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI/NheI (destroyed during cloning)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: Forw (T7): 5'-­‐TAATACGACTCACTATAGGG-­‐3'
• 3′ sequencing primer: Rev: 5'-­‐TAGAAGGCACAGTCGAGG-­‐3'

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry