MK969
(Plasmid
#71432)
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PurposeThe pAK plasmid expresses GFP driven by a T7 promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 71432 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAK
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberUnknown
Gene/Insert
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Gene/Insert namegfp
- Promoter T7
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7
- 3′ sequencing primer T7term (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MK969 was a gift from Howard Salis (Addgene plasmid # 71432 ; http://n2t.net/addgene:71432 ; RRID:Addgene_71432) -
For your References section:
A portable expression resource for engineering cross-species genetic circuits and pathways. Kushwaha M, Salis HM. Nat Commun. 2015 Jul 17;6:7832. doi: 10.1038/ncomms8832. 10.1038/ncomms8832 PubMed 26184393
