MK1274
(Plasmid
#71431)
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PurposeThis E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 300 and a TetR translation rate of 35149.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 71431 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRS316
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMixed Feedback Loop version of the UBER system
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MutationT7 RBS:AACCGAGCCCAATATAGGACTCAGGGTGCCAAAAAA and TetR RBS: GCACCAGCACGCGCCGAATTCTAAGGGCGGTCAAAA
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer yCyc1proF1
- 3′ sequencing primer rrnB-T1-term-Rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please review publication for additional construct information
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MK1274 was a gift from Howard Salis (Addgene plasmid # 71431 ; http://n2t.net/addgene:71431 ; RRID:Addgene_71431) -
For your References section:
A portable expression resource for engineering cross-species genetic circuits and pathways. Kushwaha M, Salis HM. Nat Commun. 2015 Jul 17;6:7832. doi: 10.1038/ncomms8832. 10.1038/ncomms8832 PubMed 26184393
