CYN2/150 (E. coli Entner-Doudoroff v11)
(Bacterial strain
#71420)
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PurposeThis E. coli MG1655 strain has a genome-integrated genetic module containing a rationally engineered 5-enzyme Entner-Doudoroff pathway with an improved NADPH regeneration rate. Module is Cm resistant
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Depositing Lab
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Sequence Information
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Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Bacterial Strain | 71420 | Bacteria in agar stab | 1 | $85 |
Backbone
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Vector backboneN/A
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature30°C
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Growth Strain(s)This is a strain
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Copy numberUnknown
Gene/Insert
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Gene/Insert namenone
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CYN2/150 (E. coli Entner-Doudoroff v11) was a gift from Howard Salis (Addgene plasmid # 71420) -
For your References section:
Rational design of a synthetic Entner-Doudoroff pathway for improved and controllable NADPH regeneration. Ng CY, Farasat I, Maranas CD, Salis HM. Metab Eng. 2015 May;29:86-96. doi: 10.1016/j.ymben.2015.03.001. Epub 2015 Mar 10. 10.1016/j.ymben.2015.03.001 PubMed 25769287