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PurposeTo see HBV X promoter(overlaped EnhanceI) activity for luciferase assay
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 71418 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGL3-Basic
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 3100
- Total vector size (bp) 5200
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHBV X promoter and enhancer I driving Luciferase expression
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Specieshepatitis B virus
- Promoter HBV X
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer RVprimer3
- 3′ sequencing primer LucNRev (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHBV-X/EnhI-Luc was a gift from Wang-Shick Ryu (Addgene plasmid # 71418 ; http://n2t.net/addgene:71418 ; RRID:Addgene_71418) -
For your References section:
DDX3 DEAD-box RNA helicase is a host factor that restricts hepatitis B virus replication at the transcriptional level. Ko C, Lee S, Windisch MP, Ryu WS. J Virol. 2014 Dec;88(23):13689-98. doi: 10.1128/JVI.02035-14. Epub 2014 Sep 17. 10.1128/JVI.02035-14 PubMed 25231298
Map uploaded by the depositor.
