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Addgene

pHBV-S1-Luc
(Plasmid #71416)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71416 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3-Basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3100
  • Total vector size (bp) 4884
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HBV S1 promoter driving Luciferase expression
  • Species
    hepatitis B virus
  • Promoter HBV S1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer RVprimer3
  • 3′ sequencing primer LucNRev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHBV-S1-Luc was a gift from Wang-Shick Ryu (Addgene plasmid # 71416 ; http://n2t.net/addgene:71416 ; RRID:Addgene_71416)

  • For your References section:

    DDX3 DEAD-box RNA helicase is a host factor that restricts hepatitis B virus replication at the transcriptional level. Ko C, Lee S, Windisch MP, Ryu WS. J Virol. 2014 Dec;88(23):13689-98. doi: 10.1128/JVI.02035-14. Epub 2014 Sep 17. 10.1128/JVI.02035-14 PubMed 25231298
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