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PurposeTo see the activity of HBV transcription using Luciferase assay
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 71414 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGL3-Basic
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 3100
- Total vector size (bp) 5600
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHBV core promoter, enhancer I, enhancer II and luciferase
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Speciesvirus
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Insert Size (bp)2500
- Promoter HBV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer RVprimer3
- 3′ sequencing primer LucNRev (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHBV-Luc was a gift from Wang-Shick Ryu (Addgene plasmid # 71414 ; http://n2t.net/addgene:71414 ; RRID:Addgene_71414) -
For your References section:
Stimulation of hepatitis B virus genome replication by HBx is linked to both nuclear and cytoplasmic HBx expression. Cha MY, Ryu DK, Jung HS, Chang HE, Ryu WS. J Gen Virol. 2009 Apr;90(Pt 4):978-86. doi: 10.1099/vir.0.009928-0. Epub 2009 Mar 4. 10.1099/vir.0.009928-0 PubMed 19264639
Map uploaded by the depositor.
