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Addgene

pcDNA3-intein/Src
(Plasmid #71406)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71406 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Src active kinase domain inserted with Npu DnaE intein
  • Insert Size (bp)
    1300
  • Promoter pCMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer pCMV
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-intein/Src was a gift from Huiwang Ai (Addgene plasmid # 71406 ; http://n2t.net/addgene:71406 ; RRID:Addgene_71406)

  • For your References section:

    Light activation of protein splicing with a photocaged fast intein. Ren W, Ji A, Ai HW. J Am Chem Soc. 2015 Feb 18;137(6):2155-8. doi: 10.1021/ja508597d. Epub 2015 Feb 5. 10.1021/ja508597d PubMed 25647354
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