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Addgene

pMAH2-intein/mCherry
(Plasmid #71405)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71405 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMAH2
  • Backbone manufacturer
    lab constructed
  • Backbone size w/o insert (bp) 6400
  • Total vector size (bp) 7600
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Npu DnaE Intein insertion in mCherry
  • Insert Size (bp)
    1200
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer pCMV
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMAH2-intein/mCherry was a gift from Huiwang Ai (Addgene plasmid # 71405 ; http://n2t.net/addgene:71405 ; RRID:Addgene_71405)

  • For your References section:

    Light activation of protein splicing with a photocaged fast intein. Ren W, Ji A, Ai HW. J Am Chem Soc. 2015 Feb 18;137(6):2155-8. doi: 10.1021/ja508597d. Epub 2015 Feb 5. 10.1021/ja508597d PubMed 25647354
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