pMAH2-CageCys
(Plasmid #71404)

• Purpose: Expresses tRNA and synthetase for mammalian encoding of a photocaged cysteine
• Depositing Lab: Huiwang Ai
• Publication: Ren et al J Am Chem Soc. 2015 Feb 18;137(6):2155-8. doi: 10.1021/ja508597d. Epub 2015 Feb 5.

Backbone

• Vector backbone: pMAH2
• Backbone size w/o insert (bp): 6500
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: aminoacyl-tRNA synthetase for a caged cysteine
• Insert Size (bp): 2500
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Hind III (not destroyed)
• 3′ cloning site: Pme I (not destroyed)
• 5′ sequencing primer: pCMV

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The aminoacyl-tRNA synthetase insert contains ML42GQ compared to all available reference sequences. The depositor states that these mutations are intended and the plasmid functions as described in the associated publication.