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Addgene

pCMV-DnpK
(Plasmid #71403)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71403 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    unknown
  • Total vector size (bp) 7245
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    aminoacyl-tRNA synthetase
  • Species
    Synthetic
  • Insert Size (bp)
    1200
  • Mutation
    Y271M,L274T,C313A, and Y349F
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer pCMV
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The pyrrolysyl-tRNA-synthetase insert contains Y272M, L275T, C314A and Y350Fcompared to the closest reference sequence ACB70968.1. The depositor states that these mutations are intended.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-DnpK was a gift from Huiwang Ai (Addgene plasmid # 71403 ; http://n2t.net/addgene:71403 ; RRID:Addgene_71403)

  • For your References section:

    Expanding the Genetic Code for a Dinitrophenyl Hapten. Ren W, Ji A, Wang MX, Ai HW. Chembiochem. 2015 Sep;16(14):2007-10. doi: 10.1002/cbic.201500204. Epub 2015 Jul 28. 10.1002/cbic.201500204 PubMed 26185102
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