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Addgene

pCAG-HDAC4*
(Plasmid #71402)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71402 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGEN
  • Backbone manufacturer
    Addgene plasmid #11160
  • Backbone size w/o insert (bp) 4798
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HDAC4*
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3400
  • Mutation
    After aa126, insertion leads to reading frame shift and early termination.
  • GenBank ID
    NM_006037.3
  • Entrez Gene
    HDAC4 (a.k.a. AHO3, BDMR, HA6116, HD4, HDAC-4, HDAC-A, HDACA, NEDCHF, NEDCHID)
  • Promoter CAG

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pCAG-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-HDAC4* was a gift from Bo Chen (Addgene plasmid # 71402 ; http://n2t.net/addgene:71402 ; RRID:Addgene_71402)

  • For your References section:

    A short N-terminal domain of HDAC4 preserves photoreceptors and restores visual function in retinitis pigmentosa. Guo X, Wang SB, Xu H, Ribic A, Mohns EJ, Zhou Y, Zhu X, Biederer T, Crair MC, Chen B. Nat Commun. 2015 Aug 14;6:8005. doi: 10.1038/ncomms9005. 10.1038/ncomms9005 PubMed 26272629
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