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PurposeHomologues recombination donor plasmid fo the TERT promotor region introducing a SV-40 driven GFP marker in the TERT promotor. Includes 1kb of TERT coding region in right homologues arm.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 71397 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC57
- Backbone size w/o insert (bp) 2000
- Total vector size (bp) 4500
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTERT
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SpeciesH. sapiens (human)
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Entrez GeneTERT (a.k.a. CMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TP2, TRT, hEST2, hTRT)
- Promoter Endogenous TERT promotor
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Tag
/ Fusion Protein
- SV-40 GFP insertion in TERT promotor
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13f
- 3′ sequencing primer M13r (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HR-TERT-SV40-GFP was a gift from Thomas Cech (Addgene plasmid # 71397 ; http://n2t.net/addgene:71397 ; RRID:Addgene_71397) -
For your References section:
A novel two-step genome editing strategy with CRISPR-Cas9 provides new insights into telomerase action and TERT gene expression. Xi L, Schmidt JC, Zaug AJ, Ascarrunz DR, Cech TR. Genome Biol. 2015 Nov 10;16(1):231. doi: 10.1186/s13059-015-0791-1. 10.1186/s13059-015-0791-1 [pii] PubMed 26553065
Map uploaded by the depositor.
