pLVX-EF1alpha-2xGFP:NES-IRES-2xRFP:NLS
(Plasmid #71396)

• Purpose: 2Gi2R assay
• Depositing Lab: Fred Gage
• Publication: Mertens et al Cell Stem Cell. 2015 Oct 6. pii: S1934-5909(15)00408-7. doi: 10.1016/j.stem.2015.09.001.

Backbone

• Vector backbone: pLVX-EF1alpha
• Total vector size (bp): 10447
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: 2xGFP:NES
• Species: Synthetic
• Promoter: EF1alpha

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: EF1a-F

Gene/Insert 2

• Gene/Insert name: 2xRFP:NLS
• Alt name: mCherry
• Species: Synthetic
• Promoter: IRES
• Tag / Fusion Protein:
  • IRES (N terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: IRES-F

Resource Information

• Supplemental Documents:
  • Annotated GenBank file
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry