FLAG-LoxP-PURO-LoxP-SNAP-TERT
(Plasmid #71390)

• Purpose: Homologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes PURO resistance flanked by LoxP sites for selection.
• Depositing Lab: Thomas Cech
• Publication: Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi: 10.1186/s13059-015-0791-1.

Backbone

• Vector backbone: pUC57
• Backbone size w/o insert (bp): 2000
• Total vector size (bp): 6500
• Vector type: HR Donor
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TERT
• Alt name: Telomerase reverse transcriptase
• Species: H. sapiens (human)
• Insert Size (bp): 4000
• Entrez Gene: TERT (a.k.a. CMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TP2, TRT, hEST2, hTRT)
• Promoter: Endogenous TERT promotor
• Tag / Fusion Protein:
  • FLAG-SNAP (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: M13f
• 3′ sequencing primer: M13r

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry