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pTD125 GFP:POP1
(Plasmid #71387)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71387 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTD125
  • Backbone manufacturer
    D. Amberg (State University of New York Upstate Medical University, Syracuse, NY
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    POP1
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2700
  • Entrez Gene
    POP1 (a.k.a. YNL221C)
  • Promoter ACT1
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer GFP-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Vector is URA, CEN-based.
Construct has: ACT1promoter-GFP-POP1-ACT1terminator

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTD125 GFP:POP1 was a gift from Mark Schmitt (Addgene plasmid # 71387 ; http://n2t.net/addgene:71387 ; RRID:Addgene_71387)

  • For your References section:

    A specialized processing body that is temporally and asymmetrically regulated during the cell cycle in Saccharomyces cerevisiae. Gill T, Aulds J, Schmitt ME. J Cell Biol. 2006 Apr 10;173(1):35-45. Epub 2006 Apr 3. 10.1083/jcb.200512025 PubMed 16585272
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