pTG005
(Plasmid #71386)

• Purpose: localization of yeast RNase RMP1 (RNase MRP binding protein) in yeast
• Depositing Lab: Mark Schmitt
• Publication: Gill et al J Cell Biol. 2006 Apr 10;173(1):35-45. Epub 2006 Apr 3.

Backbone

• Vector backbone: pTD125
• Backbone manufacturer: D. Amberg (State University of New York Upstate Medical University, Syracuse, NY
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: RMP1
• Species: S. cerevisiae (budding yeast)
• Entrez Gene: RMP1 (a.k.a. YLR145W)
• Promoter: ACT1
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: GFP-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid is also called pTD125 GFP:RMP1
Vector is URA, CEN-based.
Construct has: ACT1promoter-GFP-RMP1-ACT1terminator