Grp1-PH pGEX2TK
(Plasmid
#71379)
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Purposebacterial expression of PH domain for phosphoinositide binding assay
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 71379 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX-2TK
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Backbone manufacturerAmersham-Pharmacia
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameGrp1-PH
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SpeciesM. musculus (mouse)
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MutationPleckstrin homology (PH) domain; aa 264-391
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Entrez GeneCyth3 (a.k.a. AI648983, ARNO3, CLM3, Grp1, Pscd3)
- Promoter tac
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pGEX5'
- 3′ sequencing primer pGEX3' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Grp1-PH pGEX2TK was a gift from Mark Lemmon (Addgene plasmid # 71379 ; http://n2t.net/addgene:71379 ; RRID:Addgene_71379) -
For your References section:
Specificity and promiscuity in phosphoinositide binding by pleckstrin homology domains. Kavran JM, Klein DE, Lee A, Falasca M, Isakoff SJ, Skolnik EY, Lemmon MA. J Biol Chem. 1998 Nov 13;273(46):30497-508. PubMed 9804818