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Addgene

Grp1-PH pEGFP-C1
(Plasmid #71378)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71378 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Grp1-PH
  • Species
    M. musculus (mouse)
  • Mutation
    Pleckstrin homology (PH) domain; aa 247-399
  • Entrez Gene
    Cyth3 (a.k.a. AI648983, ARNO3, CLM3, Grp1, Pscd3)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (unknown if destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Grp1-PH pEGFP-C1 was a gift from Mark Lemmon (Addgene plasmid # 71378 ; http://n2t.net/addgene:71378 ; RRID:Addgene_71378)

  • For your References section:

    Specificity and promiscuity in phosphoinositide binding by pleckstrin homology domains. Kavran JM, Klein DE, Lee A, Falasca M, Isakoff SJ, Skolnik EY, Lemmon MA. J Biol Chem. 1998 Nov 13;273(46):30497-508. PubMed 9804818
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