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Addgene

Dapp1-PH pET11a
(Plasmid #71376)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71376 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET11a
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Dapp1-PH
  • Species
    H. sapiens (human)
  • Mutation
    Pleckstrin homology (PH) domain; aa 148-273
  • Entrez Gene
    DAPP1 (a.k.a. BAM32)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BglII (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminal
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Dapp1-PH pET11a was a gift from Mark Lemmon (Addgene plasmid # 71376 ; http://n2t.net/addgene:71376 ; RRID:Addgene_71376)

  • For your References section:

    Structural basis for discrimination of 3-phosphoinositides by pleckstrin homology domains. Ferguson KM, Kavran JM, Sankaran VG, Fournier E, Isakoff SJ, Skolnik EY, Lemmon MA. Mol Cell. 2000 Aug;6(2):373-84. S1097-2765(00)00037-X [pii] PubMed 10983984
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