pRS426-GFP-Num1-PH
(Plasmid
#71374)
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PurposeFor analysis of PH domain localization in yeast
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 71374 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRS426
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Backbone manufacturerATCC
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNum1-PH
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SpeciesS. cerevisiae (budding yeast)
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MutationPleckstrin homology (PH) domain; aa2563-2692
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Tag
/ Fusion Protein
- EGFP (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRS426-GFP-Num1-PH was a gift from Mark Lemmon (Addgene plasmid # 71374 ; http://n2t.net/addgene:71374 ; RRID:Addgene_71374) -
For your References section:
Genome-wide analysis of membrane targeting by S. cerevisiae pleckstrin homology domains. Yu JW, Mendrola JM, Audhya A, Singh S, Keleti D, DeWald DB, Murray D, Emr SD, Lemmon MA. Mol Cell. 2004 Mar 12;13(5):677-88. 10.1016/S1097-2765(04)00083-8 PubMed 15023338