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Addgene

pRS426-GFP-Num1-PH
(Plasmid #71374)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71374 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pRS426
  • Backbone manufacturer
    ATCC
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Num1-PH
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    Pleckstrin homology (PH) domain; aa2563-2692
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS426-GFP-Num1-PH was a gift from Mark Lemmon (Addgene plasmid # 71374 ; http://n2t.net/addgene:71374 ; RRID:Addgene_71374)
  • For your References section:

    Genome-wide analysis of membrane targeting by S. cerevisiae pleckstrin homology domains. Yu JW, Mendrola JM, Audhya A, Singh S, Keleti D, DeWald DB, Murray D, Emr SD, Lemmon MA. Mol Cell. 2004 Mar 12;13(5):677-88. 10.1016/S1097-2765(04)00083-8 PubMed 15023338