pGEX2TK-Skm1-PH
(Plasmid
#71371)
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Purposebacterial expression of PH domain for phosphoinositide binding assay
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 71371 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX-2TK
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Backbone manufacturerAmersham-Pharmacia
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSkm1-PH
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SpeciesS. cerevisiae (budding yeast)
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MutationPleckstrin homology (PH) domain; aa1-132
- Promoter tac
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pGEX5'
- 3′ sequencing primer pGEX3' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX2TK-Skm1-PH was a gift from Mark Lemmon (Addgene plasmid # 71371 ; http://n2t.net/addgene:71371 ; RRID:Addgene_71371) -
For your References section:
Genome-wide analysis of membrane targeting by S. cerevisiae pleckstrin homology domains. Yu JW, Mendrola JM, Audhya A, Singh S, Keleti D, DeWald DB, Murray D, Emr SD, Lemmon MA. Mol Cell. 2004 Mar 12;13(5):677-88. 10.1016/S1097-2765(04)00083-8 PubMed 15023338