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csd6_13-330_C176A/pET28b(+) N-tag
(Plasmid #71360)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71360 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5300
  • Total vector size (bp) 6200
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    To produce protein, transform into Rosetta2(DE3)pLysS cells. induction: 18 degree @ 20 hours
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    HP0518
  • Alt name
    Csd6
  • Species
    changed Cysteine 176 to Alanine
  • Insert Size (bp)
    954
  • GenBank ID
  • Entrez Gene
    HP0518 (a.k.a. HP0518)
  • Promoter T7
  • Tag / Fusion Protein
    • Histidine tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    csd6_13-330_C176A/pET28b(+) N-tag was a gift from SeWon Suh (Addgene plasmid # 71360 ; http://n2t.net/addgene:71360 ; RRID:Addgene_71360)

  • For your References section:

    The Cell Shape-determining Csd6 Protein from Helicobacter pylori Constitutes a New Family of l,d-Carboxypeptidase. Kim HS, Im HN, An DR, Yoon JY, Jang JY, Mobashery S, Hesek D, Lee M, Yoo J, Cui M, Choi S, Kim C, Lee NK, Kim SJ, Kim JY, Bang G, Han BW, Lee BI, Yoon HJ, Suh SW. J Biol Chem. 2015 Oct 9;290(41):25103-17. doi: 10.1074/jbc.M115.658781. Epub 2015 Aug 25. 10.1074/jbc.M115.658781 PubMed 26306031
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