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Addgene

pCASCADE-gltA1-gltA2
(Plasmid #71348)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71348 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCASCADE
  • Backbone manufacturer
    None - Constructed in House
  • Backbone size w/o insert (bp) 2500
  • Total vector size (bp) 2905
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    gltA1-gltA2 guide array
  • gRNA/shRNA sequence
    3 Guide Array : E . coli gltA1 and gltA2 gene promoters
  • Species
    Synthetic
  • Promoter E . coli ugpB gene promoter, low phosphate induction

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GGGAGACCACAACGG
  • 3′ sequencing primer AGTCCAGCTTGGAGCG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCASCADE-gltA1-gltA2 was a gift from Michael Lynch (Addgene plasmid # 71348 ; http://n2t.net/addgene:71348 ; RRID:Addgene_71348)

  • For your References section:

    Two-stage dynamic deregulation of metabolism improves process robustness & scalability in engineered E. coli. Ye Z, Li S, Hennigan JN, Lebeau J, Moreb EA, Wolf J, Lynch MD. Metab Eng. 2021 Nov;68:106-118. doi: 10.1016/j.ymben.2021.09.009. Epub 2021 Sep 30. 10.1016/j.ymben.2021.09.009 PubMed 34600151
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