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Purpose(Empty Backbone) Donor plasmid - kanamycin resistant donor cassette
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 71320 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepTKDP-neo
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Backbone manufacturerThomas Kuhlman
- Backbone size (bp) 5846
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Vector typeBacterial Expression, Synthetic Biology
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTKDP-neo was a gift from Thomas Kuhlman (Addgene plasmid # 71320 ; http://n2t.net/addgene:71320 ; RRID:Addgene_71320) -
For your References section:
An Integrated System for Precise Genome Modification in Escherichia coli. Tas H, Nguyen CT, Patel R, Kim NH, Kuhlman TE. PLoS One. 2015 Sep 2;10(9):e0136963. doi: 10.1371/journal.pone.0136963. eCollection 2015. PONE-D-14-57436 [pii] PubMed 26332675
Map uploaded by the depositor.
