pTRK669
(Plasmid #71313)

• Purpose: (Empty Backbone) Helper plasmid for pORI plasmids. Chloramphenicol resistant, temperature sensitive replication in E. coli and gram positives. Encodes RepA.
• Depositing Labs: Rodolphe Barrangou, Todd Klaenhammer
• Publication: Russell et al Appl Environ Microbiol. 2001 Sep;67(9):4361-4.

Backbone

• Vector backbone: pGK12
• Backbone size (bp): 4378
• Modifications to backbone: removal of erythromycin resistance gene
• Vector type: Bacterial Expression ; E. coli-gram positive bacteria shuttle vector

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Growth instructions: Chloramphenicol selection is 15 micrograms/mL in E.coli
• Copy number: Low Copy

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

In plasmid preparations from E. coli, a smaller deletion derivative of pTRK669 of about 1.7 kb may be noticeable. This smaller plasmid does not affect the functionality of pTRK669 in gram positive hosts and may not appear in those backgrounds. Addgene's quality control sequence shows the expected deletion from the smaller derivative of pTRK669.

Whole plasmid sequencing from the depositor showed deletion of the NheI site. Please see the provided digest result for reference.