TC9nickpAB
(Plasmid
#71311)
-
Purposeused as a template for in vitro transcription of Cas9 nickase mRNA after linearizing with BbsI
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 71311 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonePCR2-1 TOPO
-
Backbone manufacturerThermo-Fisher
- Backbone size w/o insert (bp) 3900
- Total vector size (bp) 8114
-
Vector typein vitro transcription
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameCas9 nickase
-
SpeciesS. pyogenes
-
MutationD10A nickase
- Promoter T7
-
Tags
/ Fusion Proteins
- NLS (N terminal on insert)
- NLS (C terminal on insert)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The poly A tail included in the sequence is sufficient for purification of full length transcript using an oligo dT column. This decreases the amount of incomplete transcripts (and therefore decreases truncated Cas9 proteins).
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
TC9nickpAB was a gift from Karl Degenhardt (Addgene plasmid # 71311 ; http://n2t.net/addgene:71311 ; RRID:Addgene_71311)
Map uploaded by the depositor.
