pBABEpuro-HA-p62-LIR
(Plasmid #71306)

• Purpose: Retroviral expression of p62 with W338A point mutation
• Depositing Lab: Jayanta Debnath
• Publication: Chen et al Oncogene. 2013 May 16;32(20):2543-54. doi: 10.1038/onc.2012.277. Epub 2012 Jul 9.

Backbone

• Vector backbone: pBABE-puro
• Backbone manufacturer: Addgene plasmid # 1764
• Backbone size w/o insert (bp): 5169
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Sequestosome-1
• Alt name: p62
• Alt name: SQSTM1
• Species: H. sapiens (human)
• Mutation: Mutated Tryptophan at position 338 to Alanine (W338A)
• Entrez Gene: SQSTM1 (a.k.a. A170, DMRV, EBIAP, FTDALS3, NADGP, OSIL, PDB3, ZIP3, p60, p62, p62B)
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: pBABE 5'
• 3′ sequencing primer: pBABE 3'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Human p62 cDNA was a kind gift from Dr. T. Johansen.
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid contains a T375A mutation within the Sequestosome-1 translation compared to NP_003891.1. The depositor states that this mutation should not affect plasmid function.