pPNT6-eGFP
(Plasmid
#71303)
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Purpose(Empty Backbone) This vector can be used for homologous recombination and allows to obtain recombinant neomycin resistant fluorescent cells that can loose fluorescence after Cre expression
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 71303 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepPNT6
- Backbone size (bp) 9102
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Modifications to backboneenhanced green fluorescent protein
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Vector typeMammalian Expression, Cre/Lox
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Resource Information
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A portion of this plasmid was derived from a plasmid made byThis plasmid was generated by Dr. Marco Musso. The GFP sequence in plasmid pPNT6 was obtained by PCR using pcDNA3-eGFP. Pcr product was cloned into bluescript. The polylinker region between the promoter and the eGFP coding region was destroyed.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPNT6-eGFP was a gift from Gian Luca Forni & Marco Musso (Addgene plasmid # 71303 ; http://n2t.net/addgene:71303 ; RRID:Addgene_71303)
