pCLX-CHOP-dGFP
(Plasmid #71299)

• Purpose: Lentiviral plasmid to express destabilized GFP under the control of the ATF-responsive CHOP promoter
• Depositing Lab: Patrick Salmon
• Publication: Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi: 10.1016/j.celrep.2015.01.061. Epub 2015 Feb 26.

Backbone

• Vector backbone: pCLX
• Backbone manufacturer: Patrick Salmon
• Backbone size w/o insert (bp): 5000
• Total vector size (bp): 8177
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: dGFP
• Insert Size (bp): 700
• Promoter: CHOP (ATF-responsive)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CGGATCTCGACGGTATCG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Bisei Ohkawara and Christof Niehrs originally described the promoter.
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Bisei Ohkawara recloned the CHOP promoter for me after a mix-up with the original plasmids. I received the good plasmids from Japan in June 2014.
from:
Division of Neurogenetics,
Center for Neurological Diseases and Cancer,
Nagoya University Graduate School of Medicine
Institute for advanced research Nagoya University (IAR)
65 Tsurumai, Showa-ku, Nagoya 466-8550, Japan.