pcDNA GASTM-3-RVG-10-Lamp2b-HA
(Plasmid #71295)

• Purpose: Encodes (N to C): GASTM mutated glycosylation motif, 3 residue spacer, RVG peptide,10 residue spacer, Lamp2b (exosomal membrane protein), 3 residue spacer, HA tag.
• Depositing Lab: Joshua Leonard
• Publication: Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72. doi: 10.1074/jbc.M114.621383. Epub 2015 Feb 5.

Backbone

• Vector backbone: pcDNA 3.1+ Hygro
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 5578
• Total vector size (bp): 6925
• Modifications to backbone: some restriction sites removed from MCS
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Lamp2b
• Species: H. sapiens (human)
• Entrez Gene: LAMP2 (a.k.a. CD107b, DND, LAMP-2, LAMPB, LGP-96, LGP110)
• Promoter: CMV
• Tags / Fusion Proteins:
  • Lamp2 signal peptide (N terminal on insert)
  • GASTM mutated glycosylation motif (N terminal on insert)
  • Rabies Viral Glycoprotein (RVG) peptide targeting acetycholine receptor (N terminal on insert)
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (destroyed during cloning)
• 3′ cloning site: BamHI (destroyed during cloning)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Supplemental Documents:
  • Plasmid Map
• A portion of this plasmid was derived from a plasmid made by: Lamp2b cDNA was purchased from Open Biosystems

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry