AgAK
(Plasmid
#71290)
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PurposeExpression of A. gambiae adenosine kinase
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 71290 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDEST14
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Backbone manufacturerInvitrogen
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAdenosine Kinase
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Alt nameAK
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Alt nameAdK
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SpeciesSynthetic; A. gambiae
- Promoter T7
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Tag
/ Fusion Protein
- 6X Histidine (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer T7 fwd
- 3′ sequencing primer T7 rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AgAK was a gift from Vern Schramm (Addgene plasmid # 71290 ; http://n2t.net/addgene:71290 ; RRID:Addgene_71290) -
For your References section:
A high-affinity adenosine kinase from Anopheles gambiae. Cassera MB, Ho MC, Merino EF, Burgos ES, Rinaldo-Matthis A, Almo SC, Schramm VL. Biochemistry. 2011 Mar 22;50(11):1885-93. doi: 10.1021/bi101921w. Epub 2011 Feb 15. 10.1021/bi101921w PubMed 21247194