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Addgene

pETa(+) HpCsd3 (42-403)
(Plasmid #71285)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71285 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21a(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5443
  • Total vector size (bp) 6529
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    To produce protein, transform into Rosetta2(DE3)pLysS cells.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    HP0506
  • Insert Size (bp)
    1086
  • Entrez Gene
    HP0506 (a.k.a. HP0506)
  • Promoter T7
  • Tag / Fusion Protein
    • His tag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETa(+) HpCsd3 (42-403) was a gift from SeWon Suh (Addgene plasmid # 71285 ; http://n2t.net/addgene:71285 ; RRID:Addgene_71285)

  • For your References section:

    Structure of Csd3 from Helicobacter pylori, a cell shape-determining metallopeptidase. An DR, Kim HS, Kim J, Im HN, Yoon HJ, Yoon JY, Jang JY, Hesek D, Lee M, Mobashery S, Kim SJ, Lee BI, Suh SW. Acta Crystallogr D Biol Crystallogr. 2015 Mar;71(Pt 3):675-86. doi: 10.1107/S1399004715000152. Epub 2015 Feb 26. 10.1107/S1399004715000152 PubMed 25760614
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