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Addgene

STIMATE(1-240)
(Plasmid #71280)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71280 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmCherry-N1
  • Backbone size w/o insert (bp) 4722
  • Total vector size (bp) 5456
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    STIMATE (1-240)
  • Alt name
    TMEM110
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    896
  • GenBank ID
    NM_198563.2
  • Entrez Gene
    TMEM110
  • Promoter CMV
  • Tag / Fusion Protein
    • mcherry (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer mcherry-R
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    STIMATE(1-240) was a gift from Yubin Zhou (Addgene plasmid # 71280 ; http://n2t.net/addgene:71280 ; RRID:Addgene_71280)

  • For your References section:

    Proteomic mapping of ER-PM junctions identifies STIMATE as a regulator of Ca(2+) influx. Jing J, He L, Sun A, Quintana A, Ding Y, Ma G, Tan P, Liang X, Zheng X, Chen L, Shi X, Zhang SL, Zhong L, Huang Y, Dong MQ, Walker CL, Hogan PG, Wang Y, Zhou Y. Nat Cell Biol. 2015 Oct;17(10):1339-47. doi: 10.1038/ncb3234. Epub 2015 Aug 31. 10.1038/ncb3234 PubMed 26322679
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