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Addgene

p221z-erTagRFP
(Plasmid #71266)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71266 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR/Zeo
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Gateway

Growth in Bacteria

  • Bacterial Resistance(s)
    Bleocin (Zeocin), 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TagRFP
  • Insert Size (bp)
    700
  • Tags / Fusion Proteins
    • signal peptide (ER) derived from an Arabidopsis vacuolar basic chitinase (N terminal on insert)
    • ER retention signal HDEL (C terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p221z-erTagRFP was a gift from Ari Pekka Mähönen (Addgene plasmid # 71266 ; http://n2t.net/addgene:71266 ; RRID:Addgene_71266)

  • For your References section:

    MultiSite Gateway compatible cell type-specific gene inducible system for plants. Siligato R, Wang X, Yadav SR, Lehesranta S, Ma G, Ursache R, Sevilem I, Zhang J, Gorte M, Prasad K, Wrzaczek M, Heidstra R, Murphy A, Scheres B, Mahonen AP. Plant Physiol. 2015 Dec 7. pii: pp.01246.2015. 10.1104/pp.15.01246 PubMed 26644504
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