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Addgene

p221k-erTq2CFP
(Plasmid #71264)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71264 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR221
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Gateway

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tq2CFP
  • Alt name
    turquoise2
  • Insert Size (bp)
    700
  • Tags / Fusion Proteins
    • signal peptide (ER) derived from an Arabidopsis vacuolar basic chitinase (N terminal on insert)
    • ER retention signal HDEL (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please, note that there is no stop codon after the
ER retention signal HDEL, and therefore, it is advisable to use a
terminator in the 3rd box containing stop adjacent to the attR2 site
(such as p2R3a-nosT or p2R3a-3AT)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p221k-erTq2CFP was a gift from Ari Pekka Mähönen (Addgene plasmid # 71264 ; http://n2t.net/addgene:71264 ; RRID:Addgene_71264)

  • For your References section:

    MultiSite Gateway compatible cell type-specific gene inducible system for plants. Siligato R, Wang X, Yadav SR, Lehesranta S, Ma G, Ursache R, Sevilem I, Zhang J, Gorte M, Prasad K, Wrzaczek M, Heidstra R, Murphy A, Scheres B, Mahonen AP. Plant Physiol. 2015 Dec 7. pii: pp.01246.2015. 10.1104/pp.15.01246 PubMed 26644504
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