pBSJL1
(Plasmid
#71261)
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Purpose(Empty Backbone) E. coli-Veillonella shuttle plasmid
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 71261 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepVJL1
- Backbone size (bp) 10000
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Vector typeBacterial Expression ; E. coli-Veillonella shuttle plasmid
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Selectable markersTet
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The 0.21-kb promoter region of gyrA and the 2.0-kb coding region of tetM were PCR generated and digested with PstI and BamHI and cloned into pBluescript II KS to create pBS-gtetM. pBS-gtetM was linearized with EcoRI and inserted into the EcoRI site of pVJL1 to generate pBSJL1.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBSJL1 was a gift from Felicia Qi (Addgene plasmid # 71261 ; http://n2t.net/addgene:71261 ; RRID:Addgene_71261) -
For your References section:
Establishment of a tractable genetic transformation system in Veillonella spp. Liu J, Xie Z, Merritt J, Qi F. Appl Environ Microbiol. 2012 May;78(9):3488-91. doi: 10.1128/AEM.00196-12. Epub 2012 Feb 17. 10.1128/AEM.00196-12 PubMed 22344660