pETM6-HpaBC
(Plasmid #71260)

• Purpose: Expresses E. coli HpaBC in monocistronic form from ePathBrick vector, pETM6
• Depositing Lab: Mattheos Koffas
• Publication: Jones et al Biotechnol Prog. 2015 Oct 21. doi: 10.1002/btpr.2185.

Backbone

• Vector backbone: pETM6
• Backbone manufacturer: Addgene Plasmid # 49795
• Backbone size w/o insert (bp): 5155
• Total vector size (bp): 7381
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: HpaB
• Species: Escherichia coli
• Insert Size (bp): 1563
• Promoter: T7

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: SpeI (not destroyed)
• 5′ sequencing primer: gggaaaCATATGAAACCAGAAGATTTCCGCGCCAG
• 3′ sequencing primer: gggaaaACTAGTTATTTCAGCAGCTTATCCAGCATGTTGATATCG

Gene/Insert 2

• Gene/Insert name: HpaC
• Species: Escherichia coli
• Insert Size (bp): 513
• Promoter: T7

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: SpeI (not destroyed)
• 5′ sequencing primer: ATGCAATTAGATGAACAACGCCTGCGC
• 3′ sequencing primer: ACTAGTTAAATCGCAGCTTCCATTTCCAGCATCAC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry