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Addgene

pTK229
(Plasmid #71256)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71256 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pXPG
  • Backbone manufacturer
    Cockerill lab, Addgene plasmid 71248
  • Total vector size (bp) 6297
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Thymidine Kinase promoter
  • Species
    HSV
  • Promoter HSV Thymidine Kinase
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sacl (destroyed during cloning)
  • 3′ cloning site PvuII (destroyed during cloning)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer LucNRev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

JM109 are the cells used by the author for pXPG and its derivatives.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTK229 was a gift from Peter Cockerill (Addgene plasmid # 71256 ; http://n2t.net/addgene:71256 ; RRID:Addgene_71256)

  • For your References section:

    The human IL-3 locus is regulated cooperatively by two NFAT-dependent enhancers that have distinct tissue-specific activities. Hawwari A, Burrows J, Vadas MA, Cockerill PN. J Immunol. 2002 Aug 15;169(4):1876-86. PubMed 12165512
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