pGME
(Plasmid #71252)

• Purpose: luciferase reporter with GM-CSF promoter + 717 bp Bgl II GM-CSF enhancer
• Depositing Lab: Peter Cockerill
• Publication: Cockerill et al Proc Natl Acad Sci U S A. 1999 Dec 21;96(26):15097-102.

Backbone

• Vector backbone: pGM
• Backbone manufacturer: Cockerill lab, Addgene plasmid 71250
• Total vector size (bp): 6766
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GM-CSF enhancer
• Alt name: colony stimulating factor 2 (granulocyte-macrophage)
• Species: H. sapiens (human)
• Entrez Gene: CSF2 (a.k.a. CSF, GMCSF)
• Promoter: GM-CSF
• Tag / Fusion Protein:
  • luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: BglII (not destroyed)
• 5′ sequencing primer: unknown
• 3′ sequencing primer: LucNRev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note that the 717-bp GM-CSF enhancer contains an extra G at position 118 from the sequence published in Cockerill et al. Proc Natl Acad Sci USA. 1993;90:2466–2470.

JM109 are the cells used by the author for pXPG and its derivatives.