pGM
(Plasmid #71250)

• Purpose: luciferase reporter with human GM-CSF promoter -627 to + 28
• Depositing Lab: Peter Cockerill
• Publication: Cockerill et al Proc Natl Acad Sci U S A. 1999 Dec 21;96(26):15097-102.

Backbone

• Vector backbone: pXPG
• Backbone manufacturer: Cockerill lab, Addgene plasmid 71248
• Total vector size (bp): 6766
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GM-CSF promoter -627 to +28 fragment
• Alt name: colony stimulating factor 2 (granulocyte-macrophage)
• Species: H. sapiens (human)
• Entrez Gene: CSF2 (a.k.a. CSF, GMCSF)
• Promoter: GM-CSF
• Tag / Fusion Protein:
  • luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: unknown
• 3′ sequencing primer: LucNRev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: human GM-CSF promoter was provided by Judith Gasson and subcloned from the CAT plasmid pCSFp1+ (Chan et al, PNAS 83, 8669-8673, 1986).
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Before being inserted into pXPG, the GM-CSF promoter has been through successive rounds of recloning which have added cloning sites to the ends.

JM109 are the cells used by the author for pXPG and its derivatives.